ELISpot Flex: Human IL-3 (ALP)

ELISpot Flex: Human IL-3 (ALP)

3435-2A

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Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAbIL3A
Detection mAbIL3B, biotin
Enzyme conjugateStreptavidin-ALP

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Delivery 4-9 business days

Shipping $0

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ELISpot substrate: BCIP/NBT-plus for ALP


Product specifications

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting human IL-3 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Flex: Human IL-3 (ALP)
ApplicationELISpot
AnalyteIL-3
ReactivityHuman
SpecificityThis kit is based on a matched pair of mAbs specific for human IL-3.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore at 4-8 °C upon receipt.
Shelf lifeAt least 18 months from date of receipt.
Human PBMC were incubated for 48 hours in an ELISpot plate. Cells were unstimulated (250,000 cells/well) or stimulated with either a mixture of Tetanus toxoid (TT) and anti-CD28 antibody (250,000 cells/well), or PMA/ionomycin (50,000 cells/well). The number of cells secreting IL-3 was analyzed by ELISpot.
Datasheet/Protocol
SDS

Tutorial, Published December 28, 2023

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Tutorial, Published November 7, 2022

7 common ELISpot errors and how to avoid them
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Tutorial, Published October 14, 2022

What is a positive ELISpot response?
DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial, Published September 2, 2022

Isolation, freezing, and thawing of PBMCs
This is a protocol on how to best isolate, freeze, and thaw PBMCs.
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IL-3

Analyte description

The cytokine interleukin 3 (IL-3) is mainly produced by activated T cells. IL-3 is also called multi-lineage-colony stimulating factor since it induces the growth and differentiation of many cells. IL-3 also activates mast cells, eosinophils, basophils, and macrophages.

Alternative namesInterleukin 3, IL-3, IL3, colony-stimulating factor, multi-CSF, mast cell growth factor, MULTI-CSF, MCGF
Cell typeT cell, Th2
Gene ID3562
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ELISpot Plus: Human IL-3 (ALP)